Recombinant humaan PEF1-eiwit

Penta-EF-hand domain containing 1, also known as PEF1, belongs to the penta-EF-hand protein family, which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca (2+) . Also, PEF1 proteins bind calcium and participate in a variety of calcium-dependent processes in vertebrates. Recombinant human PEF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Penta-EF-hand domain containing 1, ABP32, PEF1A

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

34.5 kDa (320aa) confirmed by MALDI-TOF

PEF1, Penta-EF-hand domain containing 1, ABP32, PEF1A, ATGP0905-10 µg, ATGP0905-20 µg, ATGP0905-50 µg, ATGP0905-100 µg, ATGP0905-250 µg, ATGP0905-500 µg, ATGP0905-1 mg, ATGP0905-10, ATGP0905-20, ATGP0905-50, ATGP0905-100, ATGP0905-250, ATGP0905-500, ATGP0905-1

Vernarecci S., et al. (2007) Mol Microbiol. 65 (4) :1122-38.; ; Kitaura Y., et al. (2002) Arch Biochem Biophys. 399 (1) :12-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl

Signal Transduction

NP_036524

Q9UBV8

Human

MASY PYRQGCPGAA GQAPGAPPGS YYPGPPNSGG QYGSGLPPGG GYGGPAPGGP YGPPAGGGPY GHPNPGMFPS GTPGGPYGGA APGGPYGQPP PSSYGAQQPG LYGQGGAPPN VDPEAYSWFQ SVDSDHSGYI SMKELKQALV NCNWSSFNDE TCLMMINMFD KTKSGRIDVY GFSALWKFIQ QWKNLFQQYD RDRSGSISYT ELQQALSQMG YNLSPQFTQL LVSRYCPRSA NPAMQLDRFI QVCTQLQVLT EAFREKDTAV QGNIRLSFED FVTMTASRML