Recombinant humaan Peroxiredoxin 6/PRDX6-eiwit

Peroxiredoxin 6, also known as PRDX6, is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell and can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Recombinant human peroxiredoxin 6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

1-Cys peroxiredoxin, 1-Cys PRX, 24 kDa protein, Acidic calcium-independent phospholipase A2, AiPLA2, Antioxidant protein 2, Glutathione-dependent peroxiredoxin Liver 2D page spot 40, Lysophosphatidylcholine acyltransferase 5, Non-selenium glutathione peroxidase, NSGPx, Red blood cells page spot 12, AOP2, KIAA0106, LPCAT-5

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is >2,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

27.1 kDa (244aa) confirmed by MALDI-TOF

Peroxiredoxin6, PRDX6, 1-Cys, aiPLA2, AOP2, NSGPx, p29, PRX, Peroxiredoxin6, PRDX6, PRDX 6, PRDX-6, AA690119, Prdx6 rs3, 1 Cys, 1 Cys peroxiredoxin, 1 Cys PRX, 1 cysPrx, 24 kDa protein, 9430088D19Rik, Acidic calcium independent phospholipase A2, Antioxidant protein 2, Aop2 rs3, Brp 12, mKIAA0106, Ciliary body glutathione peroxidase, CP 3, EC 1.11.1.15, EC 1.11.1.7, EC 3.1.1, GPx, KIAA0106, Liver 2D page spot 40, Ltw4, Lvtw 4, MGC46173, Non selenium glutathione peroxidase, NSGPx, ORF06, OTTHuMP00000032693, Peroxiredoxin6, PHGPx, Phospholipase A2 lysosomal, PLA2, Prdx5, PRX, Red blood cells page spot 12, Thiol specific antioxidant protein, ATGP0356-10 µg, ATGP0356-20 µg, ATGP0356-50 µg, ATGP0356-100 µg, ATGP0356-250 µg, ATGP0356-500 µg, ATGP0356-1 mg, ATGP0356-10, ATGP0356-20, ATGP0356-50, ATGP0356-100, ATGP0356-250, ATGP0356-500, ATGP0356-1

Davison EJ., et al. (2009) . Proteomics. 9 (18) :4284-97.; ; Wu J., et al. (2009) . Chem Res Toxicol. 22 (4) :668-75.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Iquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Redox Proteins

NP_004896

P30041

Human

MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP