Recombinant humaan PEX19-eiwit

PEX19, also known as Peroxin 19, localizes to the outer surface of peroxisomes in liver cells. It is essential for peroxisome biogenesis in Saccharomyces cerevisiae. It is apparently involved in the initial stage of peroxisome membrane assembly, before the import of matrix protein. Recombinant human PEX19 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Peroxisomal biogenesis factor 19, D1S2223E, HK33, PMP1, PMPI, PXF, PXMP1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.6 kDa (316aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PEX19, Peroxisomal biogenesis factor 19, D1S2223E, HK33, PMP1, PMPI, PXF, PXMP1, ATGP1201-10 µg, ATGP1201-20 µg, ATGP1201-50 µg, ATGP1201-100 µg, ATGP1201-250 µg, ATGP1201-500 µg, ATGP1201-1 mg, ATGP1201-10, ATGP1201-20, ATGP1201-50, ATGP1201-100, ATGP1201-250, ATGP1201-500, ATGP1201-1

James G.L. et al. (1994) J Biol Chem. 269: 14182-14190; ; Gotte K. et al. (1998) Mol. Cell Biol. 18: 616-628.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Signal Transduction

NP_002848.1

P40855

Human

MAAAEEGCSV GAEADRELEE LLESALDDFD KAKPSPAPPS TTTAPDASGP QKRSPGDTAK DALFASQEKF FQELFDSELA SQATAEFEKA MKELAEEEPH LVEQFQKLSE AAGRVGSDMT SQQEFTSCLK ETLSGLAKNA TDLQNSSMSE EELTKAMEGL GMDEGDGEGN ILPIMQSIMQ NLLSKDVLYP SLKEITEKYP EWLQSHRESL PPEQFEKYQE QHSVMCKICE QFEAETPTDS ETTQKARFEM VLDLMQQLQD LGHPPKELAG EMPPGLNFDL DALNLSGPPG ASGEQC