Recombinant humaan PFDN1-eiwit

PFDN1 is one of sixsubunits of prefoldin, a heterohexameric chaperone protein which assists in the correct folding of other proteins. This protein delivers nonnative target proteins, principally actins and tubulins, to the eukaryotic cytosolic chaperonin for facilitated folding. Defects in PFDN1 displayed phenotypes characteristic of defects in cytoskeletal function, including manifestations of ciliary dyskinesia, neuronal loss, and defects in B and T cell development and function. Recombinant human PFDN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Prefoldin subunit 1, PDF, PFD1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15kDa (130aa) confirmed by MALDI-TOF

PFDN1, Prefoldin subunit 1, PDF, PFD1, ATGP0770-10 µg, ATGP0770-20 µg, ATGP0770-50 µg, ATGP0770-100 µg, ATGP0770-250 µg, ATGP0770-500 µg, ATGP0770-1 mg, ATGP0770-10, ATGP0770-20, ATGP0770-50, ATGP0770-100, ATGP0770-250, ATGP0770-500, ATGP0770-1

Ruley HE., et al. (2008) J Immunol. 181 (1) :476-84.; ; Cowan NJ., et al (2004) J Biol Chem. 279 (6) :4196-203.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Heat Shock Proteins

NP_002613

O60925

Human

MGSSHHHHHH SSGLVPRGSH MTELQAKVID TQQKVKLADI QIEQLNRTKK HAHLTDTEIM TLVDETNMYE GVGRMFILQS KEAIHSQLLE KQKIAEEKIK ELEQKKSYLE RSVKEAEDNI REMLMARRAQ