Recombinant humaan PFDN2-eiwit

PFDN2 (Prefoldin subunit 2) is a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. This protein binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. And binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Recombinant human PFDN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Prefoldin subunit 2, PFD2, HSPC231

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18.8 kDa (174aa) confirmed by MALDI-TOF

PFDN2, Prefoldin subunit 2, PFD2, HSPC231, ATGP0774-10 µg, ATGP0774-20 µg, ATGP0774-50 µg, ATGP0774-100 µg, ATGP0774-250 µg, ATGP0774-500 µg, ATGP0774-1 mg, ATGP0774-10, ATGP0774-20, ATGP0774-50, ATGP0774-100, ATGP0774-250, ATGP0774-500, ATGP0774-1

Vainberg I.E., et al. (1998) Cell 93:863-873; ; Grebenova D., et al. (2006) Blood Cells Mol. Dis. 37 (3) :210-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Heat Shock Proteins

NP_036526

Q9UHV9

Human

MGSSHHHHHH SSGLVPRGSH MAENSGRAGK SSGSGAGKGA VSAEQVIAGF NRLRQEQRGL ASKAAELEME LNEHSLVIDT LKEVDETRKC YRMVGGVLVE RTVKEVLPAL ENNKEQIQKI IETLTQQLQA KGKELNEFRE KHNIRLMGED EKPAAKENSE GAGAKASSAG VLVS