Recombinant humaan PFDN6-eiwit

PFDN6, also known as HKE2 or PFD6, is a subunit of the heteromeric prefoldin complex that chaperones nascent actin and alpha- and beta-tubulin chains pending their transfer to the cytosolic chaperonin containing TCP1 (CCT) complex. It binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it and binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Recombinant human PFDN6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Prefoldin subunit 6, H2-KE2, HKE2, KE-2, MGC70744, PFD6

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

16.7 kDa (149aa) confirmed by MALDI-TOF

PFDN6, Prefoldin subunit 6, H2-KE2, HKE2, KE-2, MGC70744, PFD6, ATGP0966-10 µg, ATGP0966-20 µg, ATGP0966-50 µg, ATGP0966-100 µg, ATGP0966-250 µg, ATGP0966-500 µg, ATGP0966-1 mg, ATGP0966-10, ATGP0966-20, ATGP0966-50, ATGP0966-100, ATGP0966-250, ATGP0966-500, ATGP0966-1

Sowa ME., et al. (2009) Cell. 138 (2) :389-403.; ; Simons CT., et al. (2004) J Biol Chem. 279 (6) :4196-203.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Heat Shock Proteins

NP_055075

O15212

Human

MAELIQKKLQ GEVEKYQQLQ KDLSKSMSGR QKLEAQLTEN NIVKEELALL DGSNVVFKLL GPVLVKQELG EARATVGKRL DYITAEIKRY ESQLRDLERQ SEQQRETLAQ LQQEFQRAQA AKAGAPGKA