Recombinant humaan PGAM1-eiwit

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . Mutations in this protein cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphoglycerate mutase 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA, Phosphoglycerate mutase 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.9 kDa (274aa) confirmed by MALDI-TOF

PGAM1, Phosphoglycerate mutase 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA, Phosphoglycerate mutase 1, ATGP0691-10 µg, ATGP0691-20 µg, ATGP0691-50 µg, ATGP0691-100 µg, ATGP0691-250 µg, ATGP0691-500 µg, ATGP0691-1 mg, ATGP0691-10, ATGP0691-20, ATGP0691-50, ATGP0691-100, ATGP0691-250, ATGP0691-500, ATGP0691-1

Sakoda S., et al. (1988) J Biol Chem. 263 (32) :16899-905. ; ; Junien C., et al. (1982) Ann Genet. 25 (1) :25-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Cancer

NP_002620

P18669

Human

MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK