Recombinant humaan PGAM2-eiwit

PGAM2, also known as phosphoglycerate mutase 2, belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. Recombinant human PGAM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.9 kDa (273aa) confirmed by MALDI-TOF

PGAM2, Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM, ATGP1427-10 µg, ATGP1427-20 µg, ATGP1427-50 µg, ATGP1427-100 µg, ATGP1427-250 µg, ATGP1427-500 µg, ATGP1427-1 mg, ATGP1427-10, ATGP1427-20, ATGP1427-50, ATGP1427-100, ATGP1427-250, ATGP1427-500, ATGP1427-1

Tsujino S., et al. (1989) J. Biol. Chem. 264:15334-15337; ; Hadjigeorgiou G.M., et al. (1999) Neuromuscul. Disord. 9:399-402

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Enzymes & Proteases

NP_000281

P15259

Human

MATHRLVMVR HGESTWNQEN RFCGWFDAEL SEKGTEEAKR GAKAIKDAKM EFDICYTSVL KRAIRTLWAI LDGTDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEE QVKIWRRSFD IPPPPMDEKH PYYNSISKER RYAGLKPGEL PTCESLKDTI ARALPFWNEE IVPQIKAGKR VLIAAHGNSL RGIVKHLEGM SDQAIMELNL PTGIPIVYEL NKELKPTKPM QFLGDEETVR KAMEAVAAQG KAK