Recombinant humaan PGD2 Synthase/PTGDS-eiwit

Prostaglandin-H2 D-isomerase, also known as PTGDS, is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2) . It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. PTGDS is the most abundant protein in the cerebral spinal fluid and recent evidence suggests that PTGDS acts as a beta-amyloid chaperone and may have a role in the deposition of Ab plaques in Alzheimer's disease. Recombinant human PTGDS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Prostaglandin-H2 D-isomerase, L-PGDS, LPGDS, PDS, PGD2, PGDS, PGDS2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

20.9 kDa (189aa) confirmed by MALDI-TOF

PGD2 Synthase, PTGDS, Prostaglandin-H2 D-isomerase, L-PGDS, LPGDS, PDS, PGD2, PGDS, PGDS2, ATGP1010-10 µg, ATGP1010-20 µg, ATGP1010-50 µg, ATGP1010-100 µg, ATGP1010-250 µg, ATGP1010-500 µg, ATGP1010-1 mg, ATGP1010-10, ATGP1010-20, ATGP1010-50, ATGP1010-100, ATGP1010-250, ATGP1010-500, ATGP1010-1

Hoffmann A., et al. (1993) J Neurochem. 61 (2) :451-6.; ; Giacomelli S., et al. (1996) Biochem Biophys Acta. 1310 (3) :269-76.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 1mM EDTA, 0.1M NaCl

Signal Transduction

NP_000945

P41222

Human

APEAQVSVQ PNFQQDKFLG RWFSAGLASN SSWLREKKAA LSMCKSVVAP ATDGGLNLTS TFLRKNQCET RTMLLQPAGS LGSYSYRSPH WGSTYSVSVV ETDYDQYALL YSQGSKGPGE DFRMATLYSR TQTPRAELKE KFTAFCKAQG FTEDTIVFLP QTDKCMTEQ