Recombinant humaan PGK2-eiwit

PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 500unit/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

46.9 kDa (437aa) confirmed by MALDI-TOF

PGK2, Phosphoglycerate kinase 2, dJ417L20.2, PGKB, PGKPSS, ATGP3287-10 µg, ATGP3287-20 µg, ATGP3287-50 µg, ATGP3287-100 µg, ATGP3287-250 µg, ATGP3287-500 µg, ATGP3287-1 mg, ATGP3287-10, ATGP3287-20, ATGP3287-50, ATGP3287-100, ATGP3287-250, ATGP3287-500, ATGP3287-1

Svaasand EK. et al. (2007) Muscle Nerve. 36:679-84.; ; Valentin C. et al. (1998) Hum Mutat. 12 (4) :280-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl

Metabolism

NP_620061

P07205

Human

MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM