Recombinant humaan PGLS-eiwit

6-phosphogluconolactonase, also known PGLS, is an enzyme in the second step pentose phosphate pathway, which is essential for the synthesis of nucleotide sugars and NADPH, the main source of reducing power. It converts 6-phosphogluconolactone to 6-phosphogluconate. Recombinant human PGLS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

6-phosphogluconolactonase, 6PGL

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

29.7 kDa (278aa) confirmed by MALDI-TOF

PGLS, 6-phosphogluconolactonase, 6PGL, ATGP0758-10 µg, ATGP0758-20 µg, ATGP0758-50 µg, ATGP0758-100 µg, ATGP0758-250 µg, ATGP0758-500 µg, ATGP0758-1 mg, ATGP0758-10, ATGP0758-20, ATGP0758-50, ATGP0758-100, ATGP0758-250, ATGP0758-500, ATGP0758-1

Collard F., et al. (1999) FEBS Lett. 459 (2) :223-6. ; ; Hong M K., et al. (2008) Acta Crystallogr Sect F Struct Biol Cryst Commun. 64 (11) :1016-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Metabolism

NP_036220

O95336

Human

MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV PFEKHSTL