Recombinant humaan PGP-eiwit

Phosphoglycolate phosphatase, also known as PGP, is detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level) . The catalytic activity of PGP is 2-phosphoglycolate + H2O = glycolate + phosphate. The highest activities occur in skeletal muscle and cardiac muscle. Recombinant human PGP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 3,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Phosphoglycolate phosphatase

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 3,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

36.5 kDa (345aa) confirmed by MALDI-TOF

PGP, Phosphoglycolate phosphatase, ATGP3564-10 µg, ATGP3564-20 µg, ATGP3564-50 µg, ATGP3564-100 µg, ATGP3564-250 µg, ATGP3564-500 µg, ATGP3564-1 mg, ATGP3564-10, ATGP3564-20, ATGP3564-50, ATGP3564-100, ATGP3564-250, ATGP3564-500, ATGP3564-1

Barker R.F., et al. (1978) Ann. Hum. Genet. 42:143-151

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_001035830

A6NDG6

Human

MGSSHHHHHH SSGLVPRGSH MGSHMAAAEA GGDDARCVRL SAERAQALLA DVDTLLFDCD GVLWRGETAV PGAPEALRAL RARGKRLGFI TNNSSKTRAA YAEKLRRLGF GGPAGPGASL EVFGTAYCTA LYLRQRLAGA PAPKAYVLGS PALAAELEAV GVASVGVGPE PLQGEGPGDW LHAPLEPDVR AVVVGFDPHF SYMKLTKALR YLQQPGCLLV GTNMDNRLPL ENGRFIAGTG CLVRAVEMAA QRQADIIGKP SRFIFDCVSQ EYGINPERTV MVGDRLDTDI LLGATCGLKT ILTLTGVSTL GDVKNNQESD CVSKKKMVPD FYVDSIADLL PALQG