Recombinant humaan PGPEP-1-eiwit

PGPEP1 is an omega peptidase which removes pyroglutamyl residues from the amino termini of peptides and proteins. It is a cytosolic cysteine peptidase that is expressed in most cell types. The enzyme requires a thiolreducing agent for activity. PGPEP1 is potentially involved in the inactivation of biologically active peptides that possess an amino terminal pyroglutamyl group. Examples of such peptides include neurotensin, luteinizing hormone releasing hormone, and thyrotropinreleasing hormone. Recombinant human PGPEP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Pyroglutamyl-peptidase 1, Pyroglutamyl-peptidase I, Pcp, PGP, PGP-I, PGPI

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25.5 kDa (232aa) confirmed by MALDI-TOF

PGPEP1, Pyroglutamyl-peptidase 1, Pyroglutamyl-peptidase I, Pcp, PGP, PGP-I, PGPI, ATGP1839-10 µg, ATGP1839-20 µg, ATGP1839-50 µg, ATGP1839-100 µg, ATGP1839-250 µg, ATGP1839-500 µg, ATGP1839-1 mg, ATGP1839-10, ATGP1839-20, ATGP1839-50, ATGP1839-100, ATGP1839-250, ATGP1839-500, ATGP1839-1

Kilbane Z. et al. (2007) Mol. Cell. Biochem. 297:189.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Enzymes & Proteases

NP_060182

Q9NXJ5

Human

MEQPRKA VVVTGFGPFG EHTVNASWIA VQELEKLGLG DSVDLHVYEI PVEYQTVQRL IPALWEKHSP QLVVHVGVSG MATTVTLEKC GHNKGYKGLD NCRFCPGSQC CVEDGPESID SIIDMDAVCK RVTTLGLDVS VTISQDAGRY LCDFTYYTSL YQSHGRSAFV HVPPLGKPYN ADQLGRALRA IIEEMLDLLE QSEGKINYCH KH