Recombinant humaan PHLDA2-eiwit

PHLDA2, also known as Pleckstrin homology-like domain family A member 2, is a cytoplasmic protein that is involved in fetal and placental growth. It is an apoptosis-related protein that acts as a negative growth regulator and is expressed during normal human development. This protein is imprinted on placenta, liver and fetal tissues during embryogenesis and is removed once development is complete. Recombinant human PHLDA2 protein, fused to His-tag at N-terminus was expressed in E. coli and purified by using conventional chromatography techniques.

Pleckstrin homology-like domain family A member 2, BRW1C, BWR1C, HLDA2, IPL, TSSC3, Pleckstrin homology-like domain family A member 2 Beckwith Wiedemann syndrome chromosome region 1 candidate protein C, BRW 1C, BWR 1C, HLDA 2, HLDA2, Imprinted in placenta and liver, Imprinted in placenta and liver protein, p17 Beckwith Wiedemann region 1C, p17 BWR1C, PHLDA 2, Pleckstrin homology like domain family A member 2, TSSC 3, Tumor supressing STF cDNA 3, Tumor suppressing STF cDNA 3 protein, Tumor suppressing subchromosomal transferable, fragment candidate gene 3 protein, Tumor suppressing subchromosomal transferable fragment cDNA 3, Tumor suppressing subtransferable candidate 3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

19.2 kDa (172aa) confirmed by MALDI-TOF

PHLDA2, Pleckstrin homology-like domain family A member 2, BRW1C, BWR1C, HLDA2, IPL, TSSC3, Pleckstrin homology-like domain family A member 2 Beckwith Wiedemann syndrome chromosome region 1 candidate protein C, BRW 1C, BWR 1C, HLDA 2, HLDA2, Imprinted in placenta and liver, Imprinted in placenta and liver protein, p17 Beckwith Wiedemann region 1C, p17 BWR1C, PHLDA 2, Pleckstrin homology like domain family A member 2, TSSC 3, Tumor supressing STF cDNA 3, Tumor suppressing STF cDNA 3 protein, Tumor suppressing subchromosomal transferable, fragment candidate gene 3 protein, Tumor suppressing subchromosomal transferable fragment cDNA 3, Tumor suppressing subtransferable candidate 3, ATGP0546-10 µg, ATGP0546-20 µg, ATGP0546-50 µg, ATGP0546-100 µg, ATGP0546-250 µg, ATGP0546-500 µg, ATGP0546-1 mg, ATGP0546-10, ATGP0546-20, ATGP0546-50, ATGP0546-100, ATGP0546-250, ATGP0546-500, ATGP0546-1

Lee MP, et al. (1998) Cancer Res.. 58 (5) :1052-6.; ; Frank D. et al. (2000) Mamm Genome. 10 (12) :1150-9..;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Cancer

NP_003302.1

Q53GA4

Human

MKSPDEVLRE GELEKRSDSL FQLWKKKRGV LTSDRLSLFP ASPRARPKEL RFHSILKVDC VERTGKYVYF TIVTTDHKEI DFRCAGESCW NAAIALALID FQNRRALQDF RSRQERTAPA APAEDAVAAA AAAPSEPSEP SRPSPQPKPR TP