Recombinant humaan PHOSPHO1-eiwit

Phosphoethanolamine/phosphocholine phosphatase, also known as PHOSPHO1, has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho) . This protein is involved in the generation of inorganic phosphate for bone mineralization. Recombinant human PHOSPHO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphoethanolamine/phosphocholine phosphatase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

32.2 kDa (291aa) confirmed by MALDI-TOF

PHOSPHO1, Phosphoethanolamine/phosphocholine phosphatase, ATGP1974-10 µg, ATGP1974-20 µg, ATGP1974-50 µg, ATGP1974-100 µg, ATGP1974-250 µg, ATGP1974-500 µg, ATGP1974-1 mg, ATGP1974-10, ATGP1974-20, ATGP1974-50, ATGP1974-100, ATGP1974-250, ATGP1974-500, ATGP1974-1

Roberts S.J., et al. (2004) Biochem. J. 382:59-65; ; Houston B R., et al. (2004) Bone. 34:629-637;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol

Stem Cell

NP_848595

Q8TCT1

Human

MGSSHHHHHH SSGLVPRGSH MGSHMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKS C