Recombinant humaan PHPT1-eiwit

PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH 7.5 at 37C.

14 kDa phosphohistidine phosphatas, bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is >120unit/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH 7.5 at 37C.

15.9 kDa (145aa) confirmed by MALDI-TOF

PHPT1, 14 kDa phosphohistidine phosphatas, bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10, ATGP3560-10 µg, ATGP3560-20 µg, ATGP3560-50 µg, ATGP3560-100 µg, ATGP3560-250 µg, ATGP3560-500 µg, ATGP3560-1 mg, ATGP3560-10, ATGP3560-20, ATGP3560-50, ATGP3560-100, ATGP3560-250, ATGP3560-500, ATGP3560-1

Srivastava S., et al. (2008) Proc Natl Acad Sci U S A. 105 (38) :14442-6.; ; van Vlierberghe P., et al. (2006) Leukemia. 20 (7) :1245-53.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol

Enzymes & Proteases

NP_054891

Q9NRX4

Human

MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY