Recombinant humaan PIG3/TP53I3-eiwit

TP53I3, also known as quinone oxidoreductase PIG3, is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. It is localized to the cytoplasm and induced in primary, non-transformed and transformed cell cultures after exposure to genotoxic agents. This protein has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. Recombinant human TP53I3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Quinone oxidoreductase PIG3, NADPH:quinone reductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.6 kDa (352aa) confirmed by MALDI-TOF

NADPH quinone reductase PIG3, NADPH:quinone reductase PIG3, p53 induced gene 3 protein, p53-induced gene 3 protein, PIG3, Putative quinone oxidoreductase, Quinone oxidoreductase homolog, Quinone oxidoreductase PIG3, Quinone oxidoreductase PIG3 p53 induced gene 3 protein, TP53I3, Tumor protein p53 inducible protein 3, Tumor protein p53-inducible protein 3, ATGP0572-10 µg, ATGP0572-20 µg, ATGP0572-50 µg, ATGP0572-100 µg, ATGP0572-250 µg, ATGP0572-500 µg, ATGP0572-1 mg, ATGP0572-010, ATGP0572-020, ATGP0572-050, ATGP0572-100, ATGP0572-250, ATGP0572-500, ATGP0572-01M

Flatt PM. et al. (2000) Cancer Lett. 156 (1) :63-72.; ; Porte S, et al. (2009) J Biol Chem.. 284 (25) :17194-205;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl

Cancer

NP_671713

Q53FA7

Human

MLAVHFDKPG GPENLYVKEV AKPSPGEGEV LLKVAASALN RADLMQRQGQ YDPPPGASNI LGLEASGHVA ELGPGCQGHW KIGDTAMALL PGGGQAQYVT VPEGLLMPIP EGLTLTQAAA IPEAWLTAFQ LLHLVGNVQA GDYVLIHAGL SGVGTAAIQL TRMAGAIPLV TAGSQKKLQM AEKLGAAAGF NYKKEDFSEA TLKFTKGAGV NLILDCIGGS YWEKNVNCLA LDGRWVLYGL MGGGDINGPL FSKLLFKRGS LITSLLRSRD NKYKQMLVNA FTEQILPHFS TEGPQRLLPV LDRIYPVTEI QEAHKYMEAN KNIGKIVLEL PQ