Recombinant humaan pIgR-eiwit

PIgR, also known as polymeric immunoglobulin receptor, is responsible for transcytosis of soluble dimeric IgAs and immune. This protein binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, the cleavage occurs that separates the extracellular from the transmembrane segment. PIgR expression is under the strong regulation of cytokines, hormones, and pathogenic stimuli. The expression of pIgR is critically regulated by the pro-inflammatory cytokines, such as IL-1, IL-4, TNF-α, and IFN-γ. Recombinant human pIgR, fused to Histag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human IgM is coated at 2 µg/mL (100 ul/well) can bind Human pIgR. The ED50 range ≤10 µg/mL.

Polymeric immunoglobulin receptor, PIGR, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, polymeric immunoglobulin receptor precursor

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS - PAGE

Measured by its binding ability in a functional ELISA with Human IgM. The ED50 range ≤10 µg/mL.

68.9kDa (629aa)

, ATGP3978-10 µg, ATGP3978-20 µg, ATGP3978-50 µg, ATGP3978-100 µg, ATGP3978-250 µg, ATGP3978-500 µg, ATGP3978-1 mg, ATGP3978-10, ATGP3978-20, ATGP3978-50, ATGP3978-100, ATGP3978-250, ATGP3978-500, ATGP3978-1

Giffroy D, et al, (2001) Scand J Immunol. 53:56-64.; ; Kortum AN, et al, (2014) Immunogenetics. 66:267-279.; ; Yue X, et al, (2017) Hepatolgoy. 65:1948-1962.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_002635

P01833

Human

Ala

KSPIFGP EEVNSVEGNS VSITCYYPPT SVNRHTRKYW CRQGARGGCI TLISSEGYVS SKYAGRANLT NFPENGTFVV NIAQLSQDDS GRYKCGLGIN SRGLSFDVSL EVSQGPGLLN DTKVYTVDLG RTVTINCPFK TENAQKRKSL YKQIGLYPVL VIDSSGYVNP NYTGRIRLDI QGTGQLLFSV VINQLRLSDA GQYLCQAGDD SNSNKKNADL QVLKPEPELV YEDLRGSVTF HCALGPEVAN VAKFLCRQSS GENCDVVVNT LGKRAPAFEG RILLNPQDKD GSFSVVITGL RKEDAGRYLC GAHSDGQLQE GSPIQAWQLF VNEESTIPRS PTVVKGVAGG SVAVLCPYNR KESKSIKYWC LWEGAQNGRC PLLVDSEGWV KAQYEGRLSL LEEPGNGTFT VILNQLTSRD AGFYWCLTNG DTLWRTTVEI KIIEGEPNLK VPGNVTAVLG ETLKVPCHFP CKFSSYEKYW CKWNNTGCQA LPSQDEGPSK AFVNCDENSR LVSLTLNLVT RADEGWYWCG VKQGHFYGET AAVYVAVEER KAAGSRDVSL AKADAAPDEK VLDSGFREIE NKAIQDPRLF AEEKAVADTR DQADGSRASV DSGSSEEQGG SSR