Recombinant humaan PIM1-eiwit

PIM1 belongs to the Ser/Thr protein kinase family, and PIM subfamily. This protein is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AuG (CuG) and downstream AuG codons. Recombinant human Pim1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Non-specific serine/threonine protein kinase, PIM

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.4 kDa (274aa) confirmed by MALDI-TOF

PIM1, Non-specific serine/threonine protein kinase, PIM, ATGP1997-10 µg, ATGP1997-20 µg, ATGP1997-50 µg, ATGP1997-100 µg, ATGP1997-250 µg, ATGP1997-500 µg, ATGP1997-1 mg, ATGP1997-10, ATGP1997-20, ATGP1997-50, ATGP1997-100, ATGP1997-250, ATGP1997-500, ATGP1997-1

Koike N., et al. (2000) FEBS Lett. 467:17-21; ; Ionov Y., et al. (2003) Anticancer Res. 23:167-178;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Cancer

NP_002639.1

P11309

Human

YQVGPLLGS GGFGSVYSGI RVSDNLPVAI KHVEKDRISD WGELPNGTRV PMEVVLLKKV SSGFSGVIRL LDWFERPDSF VLILERPEPV QDLFDFITER GALQEELARS FFWQVLEAVR HCHNCGVLHR DIKDENILID LNRGELKLID FGSGALLKDT VYTDFDGTRV YSPPEWIRYH RYHGRSAAVW SLGILLYDMV CGDIPFEHDE EIIRGQVFFR QRVSSECQHL IRWCLALRPS DRPTFEEIQN HPWM