Recombinant humaan PIN4-eiwit

PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions. Recombinant human PIN4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isofom 2, Parvulin-14, Parvulin-17, PPIase Pin4, hEPVH, PAR14, PAR17

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

18.8 kDa (176aa) confirmed by MALDI-TOF

PIN4, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isofom 2, Parvulin-14, Parvulin-17, PPIase Pin4, hEPVH, PAR14, PAR17, ATGP0998-10 µg, ATGP0998-20 µg, ATGP0998-50 µg, ATGP0998-100 µg, ATGP0998-250 µg, ATGP0998-500 µg, ATGP0998-1 mg, ATGP0998-10, ATGP0998-20, ATGP0998-50, ATGP0998-100, ATGP0998-250, ATGP0998-500, ATGP0998-1

Uchida T., et al. (1999) FEBS Lett. 446:278-282; ; Fujiyama-Nakamura S., et al. (2009) Mol. Cell. Proteomics. 8:1552-1565

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1mM PMSF

Metabolism

AAH93700

Q9Y237

Human

MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLEQFRVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK