Recombinant humaan PIN/DYNLL1-eiwit

DYNLL1, also known as DLC8 or PIN (protein inhibitor of neuronal nitric oxide synthase), has been identified as a protein that interacts with NOS1 resulting in NOS1 inhibition. Binding of this protein destabilizes NOS1 (Neuronal nitric oxide synthase) dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. DYNLL1 is a ubiquitously expressed protein that exhibits high expression in testis and moderate expression in brain. Recombinant human DYNLL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dynein light chain 1 cytoplasmic, DLC1, DLC8, DNCL1, DNCLC1, hdlc1, LC8, LC8a, MGC126137, MGC126138, PIN, 8 kDa dynein light chain, Dynein light chain LC8-type 1, Protein inhibitor of neuronal nitric oxide synthase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

12.5 kDa (109aa) confirmed by MALDI-TOF

PIN, DYNLL1, Dynein light chain 1 cytoplasmic, DLC1, DLC8, DNCL1, DNCLC1, hdlc1, LC8, LC8a, MGC126137, MGC126138, PIN, ATGP0744-10 µg, ATGP0744-20 µg, ATGP0744-50 µg, ATGP0744-100 µg, ATGP0744-250 µg, ATGP0744-500 µg, ATGP0744-1 mg, ATGP0744-10, ATGP0744-20, ATGP0744-50, ATGP0744-100, ATGP0744-250, ATGP0744-500, ATGP0744-1

Nakano H., et al. (2010) J Biol Chem. 285 (14) :10841-9.; ; Kubota T., et al. (2009) J Virol. 83 (13) :6952-6.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Cancer

NP_003737.1

P63167

Human

MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG