Recombinant humaan PIP4K2B-eiwit

PIP4K2B catalyzes the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4, 5-bisphosphate. It is a member of the phosphatidylinositol-4-phosphate 5-kinase family. The amino acid sequence of PIP4K2B does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, PIP4K2B interacts with p55 TNF receptor. Recombinant human PIP4K2B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, PI5P4KB, PIP5K2B, PIP5KIIB, PIP5KIIbeta

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

49.8 kDa (439aa) confirmed by MALDI-TOF

PIP4K2B, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, PI5P4KB, PIP5K2B, PIP5KIIB, PIP5KIIbeta, ATGP1942-10 µg, ATGP1942-20 µg, ATGP1942-50 µg, ATGP1942-100 µg, ATGP1942-250 µg, ATGP1942-500 µg, ATGP1942-1 mg, ATGP1942-10, ATGP1942-20, ATGP1942-50, ATGP1942-100, ATGP1942-250, ATGP1942-500, ATGP1942-1

Richardson JP, Wang M, et al. (2007) . Cell Signal. 19 (6) :1309-14.; ; Luoh SW, Venkatesan N, et al. (2004) . Oncogene. 23 (7) :1354-63.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_003550

P78356

Human

MSSNCTS TTAVAVAPLS ASKTKTKKKH FVCQKVKLFR ASEPILSVLM WGVNHTINEL SNVPVPVMLM PDDFKAYSKI KVDNHLFNKE NLPSRFKFKE YCPMVFRNLR ERFGIDDQDY QNSVTRSAPI NSDSQGRCGT RFLTTYDRRF VIKTVSSEDV AEMHNILKKY HQFIVECHGN TLLPQFLGMY RLTVDGVETY MVVTRNVFSH RLTVHRKYDL KGSTVAREAS DKEKAKDLPT FKDNDFLNEG QKLHVGEESK KNFLEKLKRD VEFLAQLKIM DYSLLVGIHD VDRAEQEEME VEERAEDEEC ENDGVGGNLL CSYGTPPDSP GNLLSFPRFF GPGEFDPSVD VYAMKSHESS PKKEVYFMAI IDILTPYDTK KKAAHAAKTV KHGAGAEIST VNPEQYSKRF NEFMSNILT