Recombinant humaan PIR-B eiwit

PIR, also known as pirin, is a member of the cupin superfamily. PIR is an Fe (II) -containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Recombinant human PIR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Pirin, Probable quercetin 2,3-dioxygenase PIR

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

34.3 kDa (310aa) confirmed by MALDI-TOF

PIR-B, Pirin, Probable quercetin 2,3-dioxygenase PIR, ATGP1413-10 µg, ATGP1413-20 µg, ATGP1413-50 µg, ATGP1413-100 µg, ATGP1413-250 µg, ATGP1413-500 µg, ATGP1413-1 mg, ATGP1413-10, ATGP1413-20, ATGP1413-50, ATGP1413-100, ATGP1413-250, ATGP1413-500, ATGP1413-1

Gelbman B.D., et al. (2007) Respir. Res. 8:10-10; ; Licciulli S., et al. (2010) Leukemia. 24:429-437

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Signal Transduction

NP_001018119

O00625

Human

MGSSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGRP GGFPDHPHRG FETVSYLLEG GSMAHEDFCG HTGKMNPGDL QWMTAGRGIL HAEMPCSEEP AHGLQLWVNL RSSEKMVEPQ YQELKSEEIP KPSKDGVTVA VISGEALGIK SKVYTRTPTL YLDFKLDPGA KHSQPIPKGW TSFIYTISGD VYIGPDDAQQ KIEPHHTAVL GEGDSVQVEN KDPKRSHFVL IAGEPLREPV IQHGPFVMNT NEEISQAILD FRNAKNGFER AKTWKSKIGN