Recombinant humaan PITPNA-eiwit

Phosphatidylinositol transfer protein alpha, also known PITPNA, is found in the cytoplasm, where it catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes. PITPNA is implicated in phospholipase C signaling and in the production of phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) by phosphoinositide-3-kinase. Recombinant human PITPNA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphatidylinositol transfer protein alpha isoform, PI-TP alpha, PITPN, VIB1A, PtdInsTP alpha

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33.9 kDa (290aa) confirmed by MALDI-TOF

PITPNA, Phosphatidylinositol transfer protein alpha isoform, PI-TP alpha, PITPN, VIB1A, PtdInsTP alpha, ATGP0826-10 µg, ATGP0826-20 µg, ATGP0826-50 µg, ATGP0826-100 µg, ATGP0826-250 µg, ATGP0826-500 µg, ATGP0826-1 mg, ATGP0826-10, ATGP0826-20, ATGP0826-50, ATGP0826-100, ATGP0826-250, ATGP0826-500, ATGP0826-1

Monaco M E., et al. (1998) Biochem J. 335:175-179.; ; Aikawa Y., et al. (1999) J Biol Chem. 274:20569-20577.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 1mM EDTA

Metabolism

NP_006215

Q00169

Human

MGSSHHHHHH SSGLVPRGSH MVLLKEYRVI LPVSVDEYQV GQLYSVAEAS KNETGGGEGV EVLVNEPYEK DGEKGQYTHK IYHLQSKVPT FVRMLAPEGA LNIHEKAWNA YPYCRTVITN EYMKEDFLIK IETWHKPDLG TQENVHKLEP EAWKHVEAVY IDIADRSQVL SKDYKAEEDP AKFKSIKTGR GPLGPNWKQE LVNQKDCPYM CAYKLVTVKF KWWGLQNKVE NFIHKQERRL FTNFHRQLFC WLDKWVDLTM DDIRRMEEET KRQLDEMRQK DPVKGMTADD