Recombinant humaan PITPNB eiwit

Phosphatidylinositol transfer protein beta isoform, also known PITPNB, is found in the cytoplasm, where it catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes. PITPNB mobilizes PI from the endoplasmic recticulum and regulates the release of PI from stored vesicles in the Golgi network. PITPNB is widely expressed in various tissues. Recombinant human PITPNB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphatidylinositol transfer protein beta isoform, PI-TP-beta, PtdInsTP beta, VIB1B

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

33.7 kDa (291aa) confirmed by MALDI-TOF

PITPNB, Phosphatidylinositol transfer protein beta isoform, PI-TP-beta, PtdInsTP beta, VIB1B, ATGP0778-10 µg, ATGP0778-20 µg, ATGP0778-50 µg, ATGP0778-100 µg, ATGP0778-250 µg, ATGP0778-500 µg, ATGP0778-1 mg, ATGP0778-10, ATGP0778-20, ATGP0778-50, ATGP0778-100, ATGP0778-250, ATGP0778-500, ATGP0778-1

Sequi B., et al. (2002) Biochem J. 366:23-34.; ; Rikova K., et al. (2007) Cell. 131:1190-1203.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

NP_036531

P48739

Human

MGSSHHHHHH SSGLVPRGSH MVLIKEFRVV LPCSVQEYQV GQLYSVAEAS KNETGGGEGI EVLKNEPYEK DGEKGQYTHK IYHLKSKVPA FVRMIAPEGS LVFHEKAWNA YPYCRTIVTN EYMKDDFFIK IETWHKPDLG TLENVHGLDP NTWKTVEIVH IDIADRSQVE PADYKADEDP ALFQSVKTKR GPLGPNWKKE LANSPDCPQM CAYKLVTIKF KWWGLQSKVE NFIQKQEKRI FTNFHRQLFC WIDKWIDLTM EDIRRMEDET QKELETMRKR GSVRGTSAAD V