Recombinant humaan PKA C bèta/PRKACB-eiwit

PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. Recombinant human PRKACB protein, fused to His-tag at N-terminus, was expressed in E. coli.

Protein kinase cAMP-activated catalytic subunit beta, Protein kinase, cAMP-dependent, Beta catalytic subunit, PKACb

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

48.6 kDa (421aa)

PRKACB, Protein kinase cAMP-dependent catalytic beta, Protein kinase, cAMP-dependent, catalytic, beta, PKACB, ATGP2666-10 µg, ATGP2666-20 µg, ATGP2666-50 µg, ATGP2666-100 µg, ATGP2666-250 µg, ATGP2666-500 µg, ATGP2666-1 mg, ATGP2666-10, ATGP2666-20, ATGP2666-50, ATGP2666-100, ATGP2666-250, ATGP2666-500, ATGP2666-1

Wu K.-J., et al. (2002) Oncogene. 21:7872-7882; ; Lignitto L., et al. (2011) Nat. Cell Biol. 13:412-422;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Signal Transduction

NP_891993

P22694

Human

MGSSHHHHHH SSGLVPRGSH MGSMAAYREP PCNQYTGTTT ALQKLEGFAS RLFHRHSKGT AHDQKTALEN DSLHFSEHTA LWDRSMKEFL AKAKEDFLKK WENPTQNNAG LEDFERKKTL GTGSFGRVML VKHKATEQYY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVRLEYA FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDHQGY IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEDIRVSI TEKCAKEFGE F