Recombinant humaan PKIB-eiwit

PKIB, also known as cAMP-dependent protein kinase inhibitor beta, is a member of the cAMP-dependent protein kinase inhibitor family. This protein may interact with the catalytic subunit of cAMP-dependent protein kinase and act as a competitive inhibitor. At least three alternatively spliced transcript variants encoding the same protein have been reported. Recombinant human PKIB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

CAMP-dependent protein kinase inhibitor beta, FLJ23817, PRKACN2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

10.6 kDa (98aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PKIB, cAMP-dependent protein kinase inhibitor beta, FLJ23817, PRKACN2, ATGP0988-10 µg, ATGP0988-20 µg, ATGP0988-50 µg, ATGP0988-100 µg, ATGP0988-250 µg, ATGP0988-500 µg, ATGP0988-1 mg, ATGP0988-10, ATGP0988-20, ATGP0988-50, ATGP0988-100, ATGP0988-250, ATGP0988-500, ATGP0988-1

Zheng L. et al. (2000) Biochem J., 349:403-7.; ; Scarpetta MA et al. (1991) J Biol Chem. 268:10927-31.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol0.1M NaCl

Signal Transduction

NP_861460.1

Q9C010

Human

MRTDSSKMTD VESGVANFAS SARAGRRNAL PDIQSSAATD GTSDLPLKLE ALSVKEDAKE KDEKTTQDQL EKPQNEEK