Recombinant humaan PKLR-eiwit

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity > 80 units/mg. One unit will convert 1.0 umole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.

Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity: > 80unit/mg. One unit will convert 1.0 umole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.

59.2 kDa (549aa) confirmed by MALDI-TOF

PKLR, Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK, ATGP0714-10 µg, ATGP0714-20 µg, ATGP0714-50 µg, ATGP0714-100 µg, ATGP0714-250 µg, ATGP0714-500 µg, ATGP0714-1 mg, ATGP0714-10, ATGP0714-20, ATGP0714-50, ATGP0714-100, ATGP0714-250, ATGP0714-500, ATGP0714-1

Meza NW., et al. (2009) Mol Ther. 17 (12) :2000-9.; ; Roy MF., et al. (2007) J Exp Med. 204 (12) :2949-61.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Cancer

NP_000289

P30613

Human

MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS