Recombinant humaan PKM-eiwit

PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. PKM2 interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant human PKM2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity > 25,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.

Pyruvate kinase muscle isoform M2, Pyruvate kinase M1/2, Pyruvate kinase muscle, Cytosolic thyroid hormone-binding protein, CTHBP, Pyruvate kinase 2/3, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1, THBP1, Opa-interacting protein 3, OIP3, Tumor M2-PK, Tyrosine-protein kinase PKM2, PK2, PK3, PKM2, p58

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity: > 25,000pmol/min/ug. One unit will convert 1.0pmole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.

60.1 kDa (551aa)

PKM2, pyruvate kinase muscle isoform M2, CTHBP, THBP1, p58, M2-PK, Tumor M2-PK, PK2, PK3, PKM, pyruvate kinase, muscle isoform M2 OIP3, PKM, TCB, OPA interacting protein 3, PKM2, Cytosolic thyroid hormone binding protein, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Thyroid hormone binding protein cytosolic, Tumour Type M2 Pyruvate Kinase, Tumor Type M2 Pyruvate Kinase, ATGP0429-10 µg, ATGP0429-20 µg, ATGP0429-50 µg, ATGP0429-100 µg, ATGP0429-500 µg, ATGP0429-1 mg, ATGP0429-10 µg, ATGP0429-20 µg, ATGP0429-50 µg, ATGP0429-100 µg, ATGP0429-250 µg, ATGP0429-500 µg, ATGP0429-1 mg, ATGP0429-10, ATGP0429-20, ATGP0429-50, ATGP0429-100, ATGP0429-250, ATGP0429-500, ATGP0429-1

Zwerschke W., et al. (2009) . Exp Cell Res. 315 (16) :2765-74; ; Mazurek S., et al. (2007) . Ernst Schering Found Symp Proc. (4) :99-124.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Cancer

NP_002645.3

P14618

Human

MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P