Recombinant humaan PLA2G7/PAF AH/Lp PLA2-eiwit

PLA2G7 (platelet-activating factor acetylhydrolase), also known as lipoprotein-associated phospholipase A2, is a phospholipase A2 enzyme. It is a secreted enzyme which catalyzes the degradation of platelet-activating factor to biologically inactive products. This protein is produced by inflammatory cells and hydrolyzes oxidised phospholipids in LDL. In the blood, PLA2G7 goes mainly with LDL and less than 20% is coupled with HDL. It is implicated in the development of atherosclerosis and is also a marker for cardiac disease. It might have a major physiologic effect in the presence of inflammatory bodily responses. Recombinant human PLA2G7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Platelet-activating factor acetylhydrolase precursor, Platelet-activating factor acetylhydrolase precursor, LDL-PLA2, LP-PLA2, PAFAD, PAFAH

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

47.2 kDa (418aa) confirmed by MALDI-TOF

PLA2G7, PAF_AH, Lp_PLA2, Platelet-activating factor acetylhydrolase precursor, Platelet-activating factor acetylhydrolase precursor, LDL-PLA2, LP-PLA2, PAFAD, PAFAH, ATGP2910-10 µg, ATGP2910-20 µg, ATGP2910-50 µg, ATGP2910-100 µg, ATGP2910-250 µg, ATGP2910-500 µg, ATGP2910-1 mg, ATGP2910-10, ATGP2910-20, ATGP2910-50, ATGP2910-100, ATGP2910-250, ATGP2910-500, ATGP2910-1

Tjoelker LW. et al. (1995) Nature. 374: 549-553

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_001161829

Q13093

Human

AAASFGQ TKIPRGNGPY SVGCTDLMFD HTNKGTFLRL YYPSQDNDRL DTLWIPNKEY FWGLSKFLGT HWLMGNILRL LFGSMTTPAN WNSPLRPGEK YPLVVFSHGL GAFRTLYSAI GIDLASHGFI VAAVEHRDRS ASATYYFKDQ SAAEIGDKSW LYLRTLKQEE ETHIRNEQVR QRAKECSQAL SLILDIDHGK PVKNALDLKF DMEQLKDSID REKIAVIGHS FGGATVIQTL SEDQRFRCGI ALDAWMFPLG DEVYSRIPQP LFFINSEYFQ YPANIIKMKK CYSPDKERKM ITIRGSVHQN FADFTFATGK IIGHMLKLKG DIDSNVAIDL SNKASLAFLQ KHLGLHKDFD QWDCLIEGDD ENLIPGTNIN TTNQHIMLQN SSGIEKYN