Recombinant humaan Placental Lactogeen/CSH1-eiwit

CSH1, also known as placental lactogen, is a member of the PRL/GH family. CSH1 gene is located on chromosome 17q which participates in the regulation of postnatal and intrauterine growth. This protein is produced only during preganancy and plays important role in increase of pancreatic islets. It also inhibits apoptosis of insulinoma cell lines by activating the phosphorylation of AKT protein. CSH1 activates PRLR through zinc-induced dimerization but does not interact with GHR. Recombinant human CSH1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human CSH1 stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.8 ng/mL.

Chorionic somatomammotropin hormone 1 precursor, chorionic somatomammotropin A, choriomammotropin, placental lactogen, PL, chorionic somatomammotropin-1, Lactogen, CS-1, CSA, CSMT, CHB3, hcs-1, hcs-A, CSH1

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.8 ng/mL.

23.1kDa (197aa)

, ATGP3948-10 µg, ATGP3948-20 µg, ATGP3948-50 µg, ATGP3948-100 µg, ATGP3948-250 µg, ATGP3948-500 µg, ATGP3948-1 mg, ATGP3948-10, ATGP3948-20, ATGP3948-50, ATGP3948-100, ATGP3948-250, ATGP3948-500, ATGP3948-1

Prager S., et al, (2003) Genet Test. 7:259-263.; ; Uemura S, et al, (2007) J Biol Chem. 282:8613-8621.; ; Awonuga AO, et al, (2011) Mol Reprod Dev. 78:519-528.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol

Signal Transduction, Hormones & Cytokines

NP_001308

P0DML2

Human

Val