Recombinant humaan PLSCR3-eiwit

PLSCR3 may mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. This protein may play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. It seems to play a role in apoptosis, through translocation of cardiolipin from the inner to the outer mitochondrial membrane which promotes BID recruitment and enhances tBid-induced mitochondrial damages. Recombinant human PLSCR3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Phospholipid scramblase 3, PLS3, Ca (2+) -dependent phospholipid scramblase 3, PL scramblase 3

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.9 kDa (288aa)

PLSCR3, Phospholipid scramblase 3, PLS3, Ca (2+) -dependent phospholipid scramblase 3, PL scramblase 3, ATGP2384-10 µg, ATGP2384-20 µg, ATGP2384-50 µg, ATGP2384-100 µg, ATGP2384-250 µg, ATGP2384-500 µg, ATGP2384-1 mg, ATGP2384-10, ATGP2384-20, ATGP2384-50, ATGP2384-100, ATGP2384-250, ATGP2384-500, ATGP2384-1

He Y., et al (2007), J. Cell. Biochem. 101:1210-1221; ; Shibata H., et al 2008) J. Biol. Chem. 283:9623-9632;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Metabolism

NP_001188505

Q9NRY6

Human

MGSSHHHHHH SSGLVPRGSH MGSMAGYLPP KGYAPSPPPP YPVTPGYPEP ALHPGPGQAP VPAQVPAPAP GFALFPSPGP VALGSAAPFL PLPGVPSGLE FLVQIDQILI HQKAERVETF LGWETCNRYE LRSGAGQPLG QAAEESNCCA RLCCGARRPL RVRLADPGDR EVLRLLRPLH CGCSCCPCGL QEMEVQAPPG TTIGHVLQTW HPFLPKFSIQ DADRQTVLRV VGPCWTCGCG TDTNFEVKTR DESRSVGRIS KQWGGLVREA LTDADDFGLQ FPLDLDVR