Recombinant humaan PLUNC-eiwit

BPIFA1, also known as PLuNC, belongs to the short subfamily of PLuNC family proteins and have homology only to the Nterminal domains of BPI. It is a secreted protein that is expressed in the secretory ducts and submucosal glands of tracheobronchial tissues. BPIFA1 binds to lipopolysaccharide (LPS) in nasal lavage fluid (NLF) which points to its role in the inflammatory response of the upper airways after exposure to irritants. Decreased levels of BPIFA1 occur in the NLF of smokers and people who have been exposed to reactive epoxy chemicals, indicating that long-term exposure to airway irritants impairs the production of BPIFA1 in the upper respiratory tract. Recombinant human BPIFA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

BPI fold-containing family A member 1, BPI fold-containing family A, member 1, bA49G10.5, LPLuNC3, LuNX, NASG, PLuNC, SPuRT

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

27.1 kDa (260aa) confirmed by MALDI-TOF

PLUNC, BPI fold-containing family A member 1, BPI fold-containing family A, member 1, bA49G10.5, LPLuNC3, LuNX, NASG, PLuNC, SPuRT, ATGP1908-10 µg, ATGP1908-20 µg, ATGP1908-50 µg, ATGP1908-100 µg, ATGP1908-250 µg, ATGP1908-500 µg, ATGP1908-1 mg, ATGP1908-10, ATGP1908-20, ATGP1908-50, ATGP1908-100, ATGP1908-250, ATGP1908-500, ATGP1908-1

Ghafouri B., et al. (2004) Biochem Biophys Acta. 1699:57-63.; ; Bingle C D., et al. (2002) Hum Mol Genet. 11: 937-943.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 30% glycerol

Immunology

NP_057667

Q9NP55

Human

MGSSHHHHHH SSGLVPRGSH MGSQFGGLPV PLDQTLPLNV NPALPLSPTG LAGSLTNALS NGLLSGGLLG ILENLPLLDI LKPGGGTSGG LLGGLLGKVT SVIPGLNNII DIKVTDPQLL ELGLVQSPDG HRLYVTIPLG IKLQVNTPLV GASLLRLAVK LDITAEILAV RDKQERIHLV LGDCTHSPGS LQISLLDGLG PLPIQGLLDS LTGILNKVLP ELVQGNVCPL VNEVLRGLDI TLVHDIVNML IHGLQFVIKV