Recombinant humaan PMF1-BGLAP-eiwit

This locus represents naturally occurring read-through transcription between the neighboring PMF1 (polyamine-modulated factor 1) and BGLAP (bone gamma-carboxyglutamate Gla protein) genes on chromosome 1. Alternative splicing results in multiple transcript variants encoding isoforms that share sequence identity with the upstream gene product, but they contain distinct C-termini due to frameshifts versus the downstream gene coding sequence. Recombinant human PMF1-BGLAP protein, fused to His-tag at N-terminus, was expressed in E. coli.

PMF1-BGLAP protein isoform 2, PMF1-BGLAP readthrough

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

26.2 kDa (234aa)

PMF1-BGLAP, PMF1-BGLAP protein isoform 2, PMF1-BGLAP readthrough, ATGP2417-10 µg, ATGP2417-20 µg, ATGP2417-50 µg, ATGP2417-100 µg, ATGP2417-250 µg, ATGP2417-500 µg, ATGP2417-1 mg, ATGP2417-10, ATGP2417-20, ATGP2417-50, ATGP2417-100, ATGP2417-250, ATGP2417-500, ATGP2417-1

Udeshi ND. et al. (2012) Mol Cell Proteomics. 11:148-159.; ; Strausberg RL. et al. (2002) Proc Natl Acad Sci u S A. 99:16899-16903;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Epigenomics (Transcription & Translation)

NP_001186591

U3KQ54

Human

MGSSHHHHHH SSGLVPRGSH MGSMAEASSA NLGSGCEEKR HEGSSSESVP PGTTISRVKL LDTMVDTFLQ KLVAAGSYQR FTDCYKCFYQ LQPAMTQQIY DKFIAQLQTS IREEISDIKE EGNLEAVLNA LDKIVEEGKV RKEPAWRPSG IPEKDLHSVM APYFLQQRDT LRRHVQKQEA ENQQLADAVL AGRRQVEELQ LQVQAQQQAW QVRSPAVQSP AKVQPLCPSR RAAR