Recombinant humaan PMM1-eiwit

PMM1 (Phosphomannomutase 1) is an enzyme that involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. This enzyme catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. In addition, it may be responsible for the degradation of glucose-1, 6-bisphosphate in ischemic brain. Recombinant human PMM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphomannomutase 1, Sec53, PMMH22

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.9 kDa (282aa) confirmed by MALDI-TOF

PMM1, phosphomannomutase 1, Sec53, PMMH22, ATGP0782-10 µg, ATGP0782-20 µg, ATGP0782-50 µg, ATGP0782-100 µg, ATGP0782-250 µg, ATGP0782-500 µg, ATGP0782-1 mg, ATGP0782-10, ATGP0782-20, ATGP0782-50, ATGP0782-100, ATGP0782-250, ATGP0782-500, ATGP0782-1

Veiga-da-Cunha M., et al. (2008) J Biol Chem. 283 (49) :33988-93.; ; Silvaggi N.R., et al. (2006) J. Biol. Chem. 281:14918-14926;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl, 0.1mM PMSF

Enzymes & Proteases

NP_002667

Q92871

Human

MGSSHHHHHH SSGLVPRGSH MAVTAQAARR KERVLCLFDV DGTLTPARQK IDPEVAAFLQ KLRSRVQIGV VGGSDYCKIA EQLGDGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNISP IGRSCTLEER IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY CLDSLDQDSF DTIHFFGNET SPGGNDFEIF ADPRTVGHSV VSPQDTVQRC REIFFPETAH EA