Recombinant humaan PMVK-eiwit

Phosphomevalonate kinase, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway. Recombinant human PMVK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphomevalonate kinase, HuMPMKI, PMK, PMKA, PMKASE, Phosphomevalonate kinase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

24.1 kDa (212aa) confirmed by MALDI-TOF

PMVK, Phosphomevalonate kinase, HuMPMKI, PMK, PMKA, PMKASE, Phosphomevalonate kinase, ATGP0733-10 µg, ATGP0733-20 µg, ATGP0733-50 µg, ATGP0733-100 µg, ATGP0733-250 µg, ATGP0733-500 µg, ATGP0733-1 mg, ATGP0733-10, ATGP0733-20, ATGP0733-50, ATGP0733-100, ATGP0733-250, ATGP0733-500, ATGP0733-1

Cho Y K., et al. (2001) J Biol chem. 278:4510-4515.; ; Hogenboom S., et al. (2004) J Lipid Res. 45:697-705.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Metabolism

NP_006547

Q15126

Human

MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL