Recombinant humaan PNMT-eiwit

PNMT, also known as phenylethanolamine N-methyltransferase, is an enzyme found in the adrenal medulla that catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline) . The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Recombinant PNMT protein was expressed in E. coli and purified by using conventional chromatography techniques.

Phenylethanolamine N-methyltransferase, PENT, PNMTase, Noradrenaline-N-methyltransferase, Phenylethanolamine N-methyltransferase

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.8 kDa (282aa) confirmed by MALDI-TOF

PNMT, Phenylethanolamine N-methyltransferase, PENT, PNMTase, Noradrenaline-N-methyltransferase, Phenylethanolamine N-methyltransferase, ATGP0417-10 µg, ATGP0417-20 µg, ATGP0417-50 µg, ATGP0417-100 µg, ATGP0417-250 µg, ATGP0417-500 µg, ATGP0417-1 mg, ATGP0417-10, ATGP0417-20, ATGP0417-50, ATGP0417-100, ATGP0417-250, ATGP0417-500, ATGP0417-1

Kaneda N., et al. (1998) Biochem Biophys Res Commun. 249 (2) :405-9.; ; Ji Y., et al. (2005) J. Neurochem. 95:1766-1776.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_002677

P11086

Human

MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL