Recombinant humaan PNPO-eiwit

PNPO, also known as pyridoxine-5'-phosphate oxidase, is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Recombinant human PNPO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Pyridoxine-5'-phosphate oxidase, Pyridoxine-5'-phosphate oxidase, FLJ10535, PDXPO

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.9 kDa (226aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PNPO, Pyridoxine-5'-phosphate oxidase, Pyridoxine-5'-phosphate oxidase, FLJ10535, PDXPO, ATGP0801-10 µg, ATGP0801-20 µg, ATGP0801-50 µg, ATGP0801-100 µg, ATGP0801-250 µg, ATGP0801-500 µg, ATGP0801-1 mg, ATGP0801-10, ATGP0801-20, ATGP0801-50, ATGP0801-100, ATGP0801-250, ATGP0801-500, ATGP0801-1

Ngo EO., et al. (1998), Biochemistry., 37 (21) :7741-8; ; Kang JH., et al. (2004) . Eur J Biochem., 271 (12) :2452-61

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,0.1mM PMSF

Metabolism

NP_060599

Q9NVS9

Human

DPVKQFAAW FEEAVQCPDI GEANAMCLAT CTRDGKPSAR MLLLKGFGKD GFRFFTNFES RKGKELDSNP FASLVFYWEP LNRQVRVEGP VKKLPEEEAE CYFHSRPKSS QIGAVVSHQS SVIPDREYLR KKNEELEQLY QDQEVPKPKS WGGYVLYPQV MEFWQGQTNR LHDRIVFRRG LPTGDSPLGP MTHRGEEDWL YERLAP