Recombinant humaan polymerase (RNA) III (DNA-gericht) polypeptide H

Source : Escherichia Coli. POLR3H Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (1-204 a.a.) and having a molecular mass of 25.3kDa (Molecular size on SDS-PAGE will appear higher) .POLR3H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Polymerase (RNA) III (DNA directed) Polypeptide H (POLR3H) is a member of the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA utilizing the 4 ribonucleoside triphosphates as substrates. POLR3H has a central role in sensing and curbing infection by intracellular bacteria and DNA viruses. POLR3H functions as nuclear and cytosolic DNA sensor which is involved in innate immune response.

DNA-directed RNA polymerase III subunit RPC8||RNA polymerase III subunit C8||DNA-directed RNA polymerase III subunit H||RNA polymerase III subunit 22.9 kDa subunit||RPC22.9||POLR3H||KIAA1665||RPC8.

Greater than 90.0% as determined by SDS-PAGE.

POLR3H protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

MGSSHHHHHH SSGLVPRGSH MGSMFVLVEM VDTVRIPPWQ FERKLNDSIA EELNKKLANK VVYNVGLCIC LFDITKLEDA YVFPGDGASHTKVHFRCVVF HPFLDEILIG KIKGCSPEGV HVSLGFFDDI LIPPESLQQP AKFDEAEQVW VWEYETEEGA HDLYMDTGEE IRFRVVDESF VDTSPTGPSS ADATTSSEEL PKKEAPYTLV GSISEPGLGL LSWWTSN.