Recombinant humaan POMC-eiwit

Pro-opiomelanocortin preproprotein, also known as POMC, a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. It regulates the corticosteroid production in the adrenal cortex. Also, POMC is cleaved into ten hormone chains named NPP, gamma-MSH, ACTH, alpha-MSH, CLIP, Lipotropin beta, Lipotropin gamma, beta-MSH, beta endorphin and Met-enkephalin. Defects in the gene that encodes POMC are the cause of POMC deficiency, which is characterized by red hair and adrenal insufficiency. Recombinant human POMC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Pro-opiomelanocortin preproprotein, ACTH, CLIP, LPH, MSH, NPP, POC

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

28.9 kDa (262aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

POMC, ATGP1088-10 µg, ATGP1088-20 µg, ATGP1088-50 µg, ATGP1088-100 µg, ATGP1088-250 µg, ATGP1088-500 µg, ATGP1088-1 mg, ATGP1088-10, ATGP1088-20, ATGP1088-50, ATGP1088-100, ATGP1088-250, ATGP1088-500, ATGP1088-1

Grassel S., et al. (2009) Arthritis Rheum. 60:3017-3027.; ; Belgardt B F., et al. (1992) J Physiol. 587:5305-5314.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 50% glycerol, 0.2M NaCl, 0.1mM PMSF, 100 mM Imidazole

Metabolism

NP_001030333

P01189

Human

WCLESSQCQ DLTTESNLLE CIRACKPDLS AETPMFPGNG DEQPLTENPR KYVMGHFRWD RFGRRNSSSS GSSGAGQKRE DVSAGEDCGP LPEGGPEPRS DGAKPGPREG KRSYSMEHFR WGKPVGKKRR PVKVYPNGAE DESAEAFPLE FKRELTGQRL REGDGPDGPA DDGAGAQADL EHSLLVAAEK KDEGPYRMEH FRWGSPPKDK RYGGFMTSEK SQTPLVTLFK NAIIKNAYKK GE