Recombinant humaan PON1-eiwit

PON1, also known as A esterase1, is a member of the paraoxonase family. It is an enzyme that hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is also a major anti-atherosclerotic component of high-density lipoprotein (HDL) . This protein is activated by PPAR-gamma, which increases synthesis and release of paraoxonase 1 enzyme from the liver, reducing atherosclerosis. PON1 shows a variety of atheroprotective properties by metabolizing inflammatory lipid peroxides. It has evolved to be a highly promiscuous enzyme capable of hydrolysing a wide variety of substrates such as lactones, cyclic carbonates, organophosphorus pesticides and nerve gases. Recombinant human PON1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37C.

Aromatic esterase 1, A-esterase 1, K-45, Serum aryldialkylphosphatase 1, paraoxonase 1, ESA, MVCD5, PON

HEK293

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH7.5 at 37C.

39.0kDa (346aa)

, ATGP3936-10 µg, ATGP3936-20 µg, ATGP3936-50 µg, ATGP3936-100 µg, ATGP3936-250 µg, ATGP3936-500 µg, ATGP3936-1 mg, ATGP3936-10, ATGP3936-20, ATGP3936-50, ATGP3936-100, ATGP3936-250, ATGP3936-500, ATGP3936-1

Banhela N., et al. (2020) Syst Rev. 9:109.; ; Soliman AM., et al. (2020) Eur J Med Chem. 197:112333.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Neuroscience

NP_000437.3

P27169

Human

Leu 16