Recombinant humaan Pontine/RUVBL1-eiwit

RuVBL1, also known as TIP49A, is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This complex may be required for the activation of transcriptional programs associated with oncogene and proto oncogene mediated growth induction, tumor suppressor mediated growth arrest. Recombinant RuVBL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP-54, INO80 complex subunit H, INO80H, NMP 238, Nuclear matrix protein 238, Pontin 52, RuvB like AAA ATPase 1, RuvB-like 1, RVB1, TAP54-alpha, TIH1, TIP49a, TIP60-associated protein 54-alpha

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

52.3 kDa (476aa) confirmed by MALDI-TOF

49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP 54, ECP54, ECP-54, INO80 complex subunit H, INO80H, NMP 238, NMP238, NMP-238, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, Pontin-52, RuvB like AAA ATPase 1, RuVBL 1, RuVBL1, RuVBL-1, RuvB-like 1, RVB1, TAP54-alpha, TIH1, TIP 49, TIP49, TIP-49, TIP49a, TIP60-associated protein 54-alpha, ATGP0629-10 µg, ATGP0629-20 µg, ATGP0629-50 µg, ATGP0629-100 µg, ATGP0629-250 µg, ATGP0629-500 µg, ATGP0629-1 mg, ATGP0629-010, ATGP0629-020, ATGP0629-050, ATGP0629-100, ATGP0629-250, ATGP0629-500, ATGP0629-01M

Jha S., et al. (2009) Mol Cell. 34 (5) :521-33. ; ; Haurie V., et al. (2009) Hepatology. 50 (6) :1871-83.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Epigenomics (Transcription & Translation)

NP_003698

Q9Y265

Human

MKIEEVKSTT KTQRIASHSH VKGLGLDESG LAKQAASGLV GQENAREACG VIVELIKSKK MAGRAVLLAG PPGTGKTALA LAIAQELGSK VPFCPMVGSE VYSTEIKKTE VLMENFRRAI GLRIKETKEV YEGEVTELTP CETENPMGGY GKTISHVIIG LKTAKGTKQL KLDPSIFESL QKERVEAGDV IYIEANSGAV KRQGRCDTYA TEFDLEAEEY VPLPKGDVHK KKEIIQDVTL HDLDVANARP QGGQDILSMM GQLMKPKKTE ITDKLRGEIN KVVNKYIDQG IAELVPGVLF VDEVHMLDIE CFTYLHRALE SSIAPIVIFA SNRGNCVIRG TEDITSPHGI PLDLLDRVMI IRTMLYTPQE MKQIIKIRAQ TEGINISEEA LNHLGEIGTK TTLRYSVQLL TPANLLAKIN GKDSIEKEHV EEISELFYDA KSSAKILADQ QDKYMK