Recombinant humaan POP7-eiwit

POP7 belongs to the histone-like Alba family. This protein is a component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Recombinant human POP7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribonuclease P protein subunit p20, 0610037N12Rik, RPP2, RPP20

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18.3 kDa (165aa) confirmed by MALDI-TOF

POP7, Ribonuclease P protein subunit p20, 0610037N12Rik, RPP2, RPP20, ATGP2749-10 µg, ATGP2749-20 µg, ATGP2749-50 µg, ATGP2749-100 µg, ATGP2749-250 µg, ATGP2749-500 µg, ATGP2749-1 mg, ATGP2749-10, ATGP2749-20, ATGP2749-50, ATGP2749-100, ATGP2749-250, ATGP2749-500, ATGP2749-1

Hands-Taylor, K.L., et al. (2010) Nucleic Acids Res. 38 (12), 4052-4066; ; Andersen, J.S., et al. (2005) Nature 433 (7021), 77-83;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA

Epigenomics (Transcription & Translation)

NP_005828

O75817

Human

MGSSHHHHHH SSGLVPRGSH MGSEFMAENR EPRGAVEAEL DPVEYTLRKR LPSRLPRRPN DIYVNMKTDF KAQLARCQKL LDGGARGQNA CSEIYIHGLG LAINRAINIA LQLQAGSFGS LQVAANTSTV ELVDELEPET DTREPLTRIR NNSAIHIRVF RVTPK