Recombinant humaan POU6F1-eiwit

POu6F1 belongs to the POu transcription factor family. This protein is transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3') . In the embryo, it is expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Recombinant human POu6F1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

POu domain class 6 transcription factor 1, POu domain, class 6, transcription factor 1, BRN5, MPOu, TCFB1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35 kDa (324aa) confirmed by MALDI-TOF

POU6F1, POu domain class 6 transcription factor 1, POu domain, class 6, transcription factor 1, BRN5, MPOu, TCFB1, ATGP2126-10 µg, ATGP2126-20 µg, ATGP2126-50 µg, ATGP2126-100 µg, ATGP2126-250 µg, ATGP2126-500 µg, ATGP2126-1 mg, ATGP2126-10, ATGP2126-20, ATGP2126-50, ATGP2126-100, ATGP2126-250, ATGP2126-500, ATGP2126-1

Wey E, Lyons GE, et al. (1994) . Eur J Biochem. 220 (3) :753-62.; ; Messier H, Brickner H, et al. (1993) . Mol Cell Biol. 13 (9) :5450-60.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_002693

Q14863

Human

MGSSHHHHHH SSGLVPRGSH MGSMPGISSQ ILTNAQGQVI GTLPWVVNSA SVAAPAPAQS LQVQAVTPQL LLNAQGQVIA TLASSPLPPP VAVRKPSTPE SPAKSEVQPI QPTPTVPQPA VVIASPAPAA KPSASAPIPI TCSETPTVSQ LVSKPHTPSL DEDGINLEEI REFAKNFKIR RLSLGLTQTQ VGQALTATEG PAYSQSAICR FEKLDITPKS AQKLKPVLEK WLNEAELRNQ EGQQNLMEFV GGEPSKKRKR RTSFTPQAIE ALNAYFEKNP LPTGQEITEI AKELNYDREV VRVWFCNRRQ TLKNTSKLNV FQIP