Recombinant humaan PP1 gamma/PPP1CC-eiwit

PPP1CC, also known as serine/threonine-protein phosphatase PP1-gamma catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein is involved in regulation of ionic conductances and long-term synaptic plasticity and may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 700 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP1gamma, PPP1G, PP-1G, Protein phosphatase 1 catalytic subunit gamma, PP1C, Protein phosphatase 1C catalytic subunit

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

Specific activity is > 700unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

39.1 kDa (343aa) confirmed by MALDI-TOF

PPP1SS, Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP1gamma, PPP1G, ATGP3561-10 µg, ATGP3561-20 µg, ATGP3561-50 µg, ATGP3561-100 µg, ATGP3561-250 µg, ATGP3561-500 µg, ATGP3561-1 mg, ATGP3561-10, ATGP3561-20, ATGP3561-50, ATGP3561-100, ATGP3561-250, ATGP3561-500, ATGP3561-1

Egloff M.-P., et al. (1995) J. Mol. Biol. 254:942-959; ; Choudhary C., et al. (2009) Science. 325:834-840

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 2mM DTT

Metabolism

NP_002701

P36873

Human

MADLDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE KKKPNATRPV TPPRGMITKQ AKK