Recombinant humaan PP1 katalytisch eiwit

PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10 mM of substrate.

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.2 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

Specific activity: >3,000unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10 mM of substrate.

39.7 kDa (350aa) confirmed by MALDI-TOF

PP1 Catalytic, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A, ATGP1174-10 µg, ATGP1174-20 µg, ATGP1174-50 µg, ATGP1174-100 µg, ATGP1174-250 µg, ATGP1174-500 µg, ATGP1174-1 mg, ATGP1174-10, ATGP1174-20, ATGP1174-50, ATGP1174-100, ATGP1174-250, ATGP1174-500, ATGP1174-1

He B., et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:843-848.; ; Trinkle-Mulcahy L., et al. (2001) J. Cell Sci. 114:4219-4228.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 50 mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50%glycerol

Enzymes & Proteases

NP_002699.1

P62136

Human

MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK