Recombinant humaan PP1 remmer-2-eiwit

PPP1R2 is an inhibitor of protein-phosphatase 1 and forms heterodimer with PP1. Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex. Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex. Recombinant human PPP1R2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein phosphatase inhibitor 2, Protein phosphatase 1, regulatory (inhibitor) subunit 2, IPP-2; IPP2, PPP1R2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

21 kDa (189aa) confirmed by MALDI-TOF

Regulatory subunit2, Regulatory subunit 2, Regulatory (inhibitor) subunit 2, Protein phosphatase1, Protein phosphatase inhibitor2, Protein phosphatase inhibitor 2, Protein phosphatase 1, PPP1R2, PP1 Inhibitor2, IPP-2, IPP2, IPP 2, ATGP2438-10 µg, ATGP2438-20 µg, ATGP2438-50 µg, ATGP2438-100 µg, ATGP2438-250 µg, ATGP2438-500 µg, ATGP2438-1 mg, ATGP2438-010, ATGP2438-020, ATGP2438-050, ATGP2438-100, ATGP2438-250, ATGP2438-500, ATGP2438-01M

Sanseau P., Jackson A, et al. (1994), Genome 5:490-496

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT

Signal Transduction

NP_006232

P41236

Human

MGSSHHHHHH SSGLVPRGSH MGSMAASTAS HRPIKGILKN KTSTTSSMVA SAEQPRGNVD EELSKKSQKW DEMNILATYH PADKDYGLMK IDEPSTPYHS MMGDDEDACS DTEATEAMAP DILARKLAAA EGLEPKYRIQ EQESSGEEDS DLSPEEREKK RQFEMKRKLH YNEGLNIKLA RQLISKDLH