Recombinant humaan PP1-subeenheid R4/PPP1R3B-eiwit

PPP1R3B is the catalytic subunit of the serine/theonine phosphatase, protein phosphatase-1. The protein is expressed in liver and skeletal muscle tissue and may be involved in regulating glycogen synthesis in these tissues. This gene may be a involved in type 2 diabetes and maturity-onset diabetes of the young. Alternate splicing results in multiple transcript variants that encode the same protein. Recombinant human PPP1R3B protein, fused to His-tag at N-terminus, was expressed in E. coli.

Protein phosphatase 1 regulatory subunit 3B, Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL, Protein phosphatase 1 regulatory subunit 4, PP1 subunit R4, Protein phosphatase 1 subunit GL, PTG, PPP1R4 GL, hepatic glycogen-targeting subunit G/L

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.1 kDa (308aa)

PPP1R3B, Protein phosphatase 1 regulatory subunit 3B, GL, PPP1R4, PTG, ATGP2330-10 µg, ATGP2330-20 µg, ATGP2330-50 µg, ATGP2330-100 µg, ATGP2330-250 µg, ATGP2330-500 µg, ATGP2330-1 mg, ATGP2330-10, ATGP2330-20, ATGP2330-50, ATGP2330-100, ATGP2330-250, ATGP2330-500, ATGP2330-1

Munro S., et al. (2005) Diabetes. 51:591-598; ; Ota T., et al. (2004) Nat. Genet. 36:40-45;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_001188258

Q86XI6

Human

MGSSHHHHHH SSGLVPRGSH MGSMMAVDIE YRYNCMAPSL RQERFAFKIS PKPSKPLRPC IQLSSKNEAS GMVAPAVQEK KVKKRVSFAD NQGLALTMVK VFSEFDDPLD MPFNITELLD NIVSLTTAES ESFVLDFSQP SADYLDFRNR LQADHVCLEN CVLKDKAIAG TVKVQNLAFE KTVKIRMTFD TWKSYTDFPC QYVKDTYAGS DRDTFSFDIS LPEKIQSYER MEFAVYYECN GQTYWDSNRG KNYRIIRAEL KSTQGMTKPH SGPDLGISFD QFGSPRCSYG LFPEWPSYLG YEKLGPYY