Recombinant humaan PP14/Glycodeline-eiwit

PAEP is a member of the kernel lipocalin super family whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. This protein been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestogen-associated endometrial protein. Recombinant human PAEP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glycodelin precursor, GD, GdA, GdF, GdS, PAEG, PEP, PP14, PAEP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21 kDa (185aa) confirmed by MALDI-TOF

PP14, Glycodelin, Glycodelin precursor, GD, GdA, GdF, GdS, PAEG, PEP, PP14, ATGP2007-10 µg, ATGP2007-20 µg, ATGP2007-50 µg, ATGP2007-100 µg, ATGP2007-250 µg, ATGP2007-500 µg, ATGP2007-1 mg, ATGP2007-10, ATGP2007-20, ATGP2007-50, ATGP2007-100, ATGP2007-250, ATGP2007-500, ATGP2007-1

Seppala M, Taylor RN, et al. (2002) . Endocr Rev. 23 (4) :401-30.; ; Seppala M, Koistinen H, et al. (2007) . Hum Reprod update. 13 (3) :275-87.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Development

NP_002562.2

P09466

Human

MDIPQTK QDLELPKLAG TWHSMAMATN NISLMATLKA PLRVHITSLL PTPEDNLEIV LHRWENNSCV EKKVLGEKTE NPKKFKINYT VANEATLLDT DYDNFLFLCL QDTTTPIQSM MCQYLARVLV EDDEIMQGFI RAFRPLPRHL WYLLDLKQME EPCRF