Recombinant humaan PP2A-alfa/PPP2R1A-eiwit

PPP2R1A also known as Serine/Threonine-Protein Phosphatase 2A 65 kDa regulatory subunit A alpha isoform, is a member of the PPP family of phosphatases, regulates a variety of essential cellular processes. PPP2R1A phosphorylation is an in vivo mechanism for regulation of the PP2A signaling complex and increased PP2A activity in heart failure. Recombinant human PPP2R1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein phosphatase 2 scaffold subunit Aalpha, Protein phosphatase 2A, Regulatory subunit A, alpha isoform, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Medium tumor antigen-associated 61 kDa protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PR65A, PP2A-Aalpha, PP2AA

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

> 90% by SDS-PAGE

67.7 kDa (612aa)

PPP2R1A, Serine/Threonine-Protein Phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PP2A-Aalpha, PP2AAALPHA, PR65A, ATGP3160-10 µg, ATGP3160-20 µg, ATGP3160-50 µg, ATGP3160-100 µg, ATGP3160-250 µg, ATGP3160-500 µg, ATGP3160-1 mg, ATGP3160-10, ATGP3160-20, ATGP3160-50, ATGP3160-100, ATGP3160-250, ATGP3160-500, ATGP3160-1

Yang R., et al. (2013) PLoS One. 8 (10) :e77285.; ; Li H H., et al. (2007) EMBO J. 26 (2) : 902-914.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_055040

P30153

Human

MAAADGD DSLYPIAVLI DELRNEDVQL RLNSIKKLST IALALGVERT RSELLPFLTD TIYDEDEVLL ALAEQLGTFT TLVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR VSSAVKAELR QYFRNLCSDD TPMVRRAAAS KLGEFAKVLE LDNVKSEIIP MFSNLASDEQ DSVRLLAVEA CVNIAQLLPQ EDLEALVMPT LRQAAEDKSW RVRYMVADKF TELQKAVGPE ITKTDLVPAF QNLMKDCEAE VRAAASHKVK EFCENLSADC RENVIMSQIL PCIKELVSDA NQHVKSALAS VIMGLSPILG KDNTIEHLLP LFLAQLKDEC PEVRLNIISN LDCVNEVIGI RQLSQSLLPA IVELAEDAKW RVRLAIIEYM PLLAGQLGVE FFDEKLNSLC MAWLVDHVYA IREAATSNLK KLVEKFGKEW AHATIIPKVL AMSGDPNYLH RMTTLFCINV LSEVCGQDIT TKHMLPTVLR MAGDPVANVR FNVAKSLQKI GPILDNSTLQ SEVKPILEKL TQDQDVDVKY FAQEALTVLS LA